Sequence Analysis of Whole Gene of Strain L99 for Preparation of Inactivated EHF Vaccine

Objective To analyze the sequence of whole gene and study on the molecular mechanism of epidemic hemorrhagic fever( EHF) virus strain L99 for preparing inactivated vaccine. Methods The total RNA of infected cells was extracted and amplified by RT-PCR using designed primers, then purified and cloned to T-vector. The recombinant plasmid was purified and sequenced, and the sequence of it was analyzed by DNASTAR software and compared with that of virus strains of other SEO types. Results The whole gene of strain L99 consisted of fragments L(6533bp) , M(3652bp)and S( 1764bp) coding 2151,1133 and 429 ammo acids respectively, and the homology of it to those of viruses of other SEO types reached 95.5%- 99.4%. Conclusion This paper provided a molecular basis of study on the biological characteristic and pathogenic mechanism of strain L99.