CALLUS INDUCTION OF BRINJAL BY GENOTYPE AND GROWTH REGULATORS

Abstract The investigation was carried out in Tissue Culture Laboratory of the Department of Genetics and Plant Breeding, Hajee Mohammad Danesh Science and Technology University, Dinajpur during the period from September, 2011 to January, 2012 with a view to study the effect of genotypes and growth regulators on callus induction of Brinjal ( Solanum sp.). Three genotypes of Solanum viz. Protab, Green Ball and Ghemma Begun (Local) were used to assess their regeneration ability. Leaf segments of three genotypes of Solanum were cultured on MS medium with different concentrations and combinations of hormones. Callus induction was ranged from 66.00 to 82.67% among the genotypes, Protab showed early callusing (7.533 days) with maximum rate of callus induction (82.67%). The highest percentage of callusing appeared in MS + 3 mg L -1 2,4-D + 0.05 mg L -1 BAP considering all genotypes. Based on the overall performance, the genotype Protab appeared as the best for number of callus initiation (16.53).