Biolistic Transformation for Delivering DNA into the Mitochondria

Genetic transformation is defined as a process of introducing genetic material (i.e. DNA) into a cell that results in a heritable change. Many approaches now exist that allow routine transformation. Although the biolistic procedure is not the most widely applied transformation technique, it is a key method to modify the mitochondrial DNA (mitDNA) and is an advisable method for gene transfer into various organisms and tissues that have proved recalcitrant to more conventional means.