Effect of Immobilization of the Micromycete Aspergillus ochraceus VKM-F4104D in Polymeric Carriers on the Production of the Fibrinolytic Protease Activator of Blood Plasma Protein C

The best known polymers (alginate, carrageenan, and acrylamide) were examined for their potential use as carriers of mycelium cells of Aspergillus ochraceus VKM-F4104D, a producer of fibrinolytic protease activator of protein C in human blood plasma during its deep cultivation. The morpho-cytological features of the development of A. ochraceus VKM-F4104D mycelium in granules of carrageenan and calcium alginate are shown. The conditions for the cultivation of micromycete immobilized in granules of 3.5% calcium alginate were optimized. The immobilized cells of the producer mycelium demonstrated increased stability and were able to synthesize protein-C protease-activator in blood plasma at the maximum level for 10 or more days. In addition, immobilized mycelial cells during cultivation can be used repeatedly (up to five cycles).