Primer sequence used in escherichia coli and staphylococcus aureus 23S-5S rRNA gene sequencing identification method

2015 
The invention relates to a primer sequence used in an escherichia coli and staphylococcus aureus 23S-5S rRNA gene sequencing identification method, and aims to provide the primer sequence used in the 23S-5S rRNA gene sequencing identification method for identification of escherichia coli and staphylococcus aureus pathogenic bacteria. The primer sequence used in the escherichia coli and staphylococcus aureus 23S-5S rRNA gene sequencing identification method is provided. With use of the sequence, a part of characteristic gene fragments in 23S-5S rRNA can be determined, and the sequence is used for accurately identify the escherichia coli and the staphylococcus aureus. Primers provided by the invention can be used for accurately sequencing the 23S-5S rRNA gene of the escherichia coli and the staphylococcus aureus; according to results of sequencing, and with combination of free public gene database retrieval, the two pathogenic bacteria can be accurately identified.
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