The Kinesis Expression of NF-κB and GFAP in the Cortex and Hippocampus of the Experimental Posttraumatic Epilepsy Rats

Objective:To observe the kinesis expression of NF-κB and GFAP in the cortex and Hippocampus,and discuss the mechanism of pathologic changes and epileptogenesis in the model of posttraumatic epilepsy (PTE). Methods:Ferrous chloride (FeCl2.1000 nmol) was injected into the motor cortex of rats via stereotactic technique Behavior was observed and EEG were recorded following injection. The pathologic examination was performed, including HE, Perls blue, Tumbull blue staining. The NF-κB and GFAP expression in the cortex and Hippocampus detected by immunofluorescence staining with Laser confocal scanning microscope. Results: All of the rats developed epileptiform discharges soon after FeCl2-injection. Compared with the control group, the expression of NF-κB were increased in 1 hour, decreased in 7 days later, decreased significantly in 30 days latert the expression of GFAP were increased in 7 or 14 days later, and there were no significance in 14 and 21 or 30 days later. There are some neurons and glia expressing NF-κB and GFAP at the same time. Conclusion: The intracortical injection of 1000nmol FeCl2 induced model is an ideal animal PTE model. The changes of the kinesis expression of NF-κB and GFAP in the cortex and Hippocampus concerned with the mechanism of pathologic changes and epileptogenesis in this model.