[Analysis of allele frequencies of HLA-DRB1*12:01:01G and HLA-DRB1*14:01:01G groups].

Objective To discriminate and analyze the relative frequencies of alleles in HLA-DRB1 * 12:01:01G(HLA-DRB1 * 12:01:01 /12:06/12:10/12:17) and HLA-DRB1 * 14:01:01G (DRB1 * 14:01:01/14:54) groups and assess their associations with HLA-DRB3 and HLA-DQB1 loci.Methods A total of 115 DNA samples previously typed as HLA-DRB1 * 12:01:01G and 108 samples from HLA-DRB1 * 14:01:01G were selected.DNA sequences for exons 1 to 3 of the HLA-DRB1 locus were analyzed for HLA-DRB1 * 12:01:01G,and exons 2 to 3 were analyzed for HLA-DRB1 * 14:01:01G by polymerase chain reaction sequence-based typing (PCR-SBT).Genotyping of HLA-DRB3 and HLA-DQB1 were achieved by PCRSBT.Results Among 115 samples previously typed as HLA-DRB1 * 12:01:01G,101 (87.8％) were confirmed as HLA-DRB1 * 12:01:01 and 14 (12.2％) were HLA-DRB1 * 12:10,but HLA-DRB1 * 12:06 and HLA-DRB1 * 12:17 alleles were not identified.For 108 samples previously typed as HLA-DRB1 * 14:01:01G,all were typed as HLA-DRB1 * 14:54.HLA-DRB1 * 12:01:01 was linked with HLA-DRB3 * 01:01:02 and HLA-DQB1 * 03:01,while HLA-DRB1 * 12:10 was strongly linked with HLA-DRB3 * 02:02:01 and HLA-DQB1 * 03:01.HLA-DRB1 * 14:54 was strongly linked with HLA-DRB3 * 02:02:01 and two different HLA-DQB1 * 05:02,* 05:03 alleles.Conclusion HLA-DRB1 * 12:01:01 was more prevalent than HLA-DRB1 * 12:10 in the HLA-DRB1 * 12:01:01G group,and HLA-DRB1 * 14:54 was the dominant allele for HLA-DRB1 * 14:01:01G. Key words: HLA-DRB1;  HLA-DRB1 * 12:01:01;  HLA-DRB1 * 14:54;  Sequencing