Isolation of homogeneous phospholipase A2 from human platelets

Abstract Phospholipase A 2 from human blood platelets has been purified to homogeneity by treatment of the platelet homogenate with H 2 SO 4 , followed by affinity chromatography of the extract. The platelet phospholipase is a soluble, heat-stable protein of MW 44000. The purified enzyme inhibits ADP-induced platelet aggregation.