Synthesis of gemcitabine analogues as potential anti-tumor agents

Gemcitabine analogue was designed to keep the anti-tumor activities but not to be the substrate of deoxycytidine deaminase. By coupling the 2-deoxy-2,2-difluoro sugar derivative with a bicyclic base, the gemcitabine analogue was synthesized, and its configuration was confirmed by 2D NMR studies.