A Micromethod of Chicken MLR: Technical Aspects and Genetic Control

Marianne Schou Institute for Experimental Immunology, University of Copenhagen, Denmark A micromethod of chicken mixed lymphocyte culture is described. In order to test the genetic control of the reaction, lymphocytes from the inbred G-Bl and G-B2 strains were cultured together with lymphocytes of the MHC identical chickens of a segregating population of (G-Bl × G-B2) F2 hybrids. Most of these combinations showed no response, which is in agreement with the major importance of the lymphocyte activating determinants (Lads) being controlled by the B complex (chicken MHC). A few strong reactions of MLC between B identical individuals indicated, however, that chickens in addition may have Lads controlled by genes not closely linked to the B complex.