Process for recovering tacrolimus with high purity

The present invention relates to a recovery method for the isolation and mass production of the other strains produced a less highly active toxic than tacrolimus immunosuppressant. The method step of extracting and filtering the fermentation broth obtained by culturing the genus Streptomyces strain producing the tacrolimus in a polar solvent; A step of adsorbing the filtrate to a primary purification resin is a non-ionic adsorption resin was obtained by elution; The primary extract purified concentrate with a water-insoluble polar solvent, a step of concentration by alkaline precipitation in which a non-polar solvent; A step of dissolving the precipitate to adsorb the secondary pure water connections flash column, eluting with concentrated to the aqueous solvent containing the silver salt; A water-insoluble polar solvent, the second purified concentrate in order to extract and concentrated to remove the color of the silver salt and the step of using activated carbon; And it comprises a step of crystallization with a polar solvent. The present invention, including the stepwise process was able to produce large quantities of highly pure tacrolimus. Tacrolimus