Effect of fractions isolated from Naja naja atra venom on antioxidation in animals

OBJECTIVE: To study the antioxidation of fraction F and H isolated from Naja naja atra venom on homogenate and RBC autoxidation. And to explore the effect of two fractions on the activities of antioxidation enzymes in mice. METHOD: Samples of tissues homogenates and RBC suspension were pretreated with fraction F/H, and then their generation of malondialdehyde(MDA) was examined. The effects of fraction F/H on O2-* produced by pyrogallol autoxidation and *OH produced by Cu2+ -VitC were checked. After being administrated i.p. with fraction F/H for 15 days, superoxide dismutase and hydroperoxidase in mice were measured for their activities. RESULT: Fraction F/H inhibited RBC autohemolysis and the generation of MDA decreased. The production of lipid peroxides in normal brain, liver and heart homogenate of rat and the elevation of lipid peroxides induced by cysteine and FeSO4 in homogenate were inhibited by addition of fraction F/H. The O2-* produced by autoxidation of pyrogallol and the *OH produced by Cu2+ -VitC system could be scavenged by fraction F/H (the potency of scavenging oxygen free radical is that fraction H > fraction F). Fraction F/H all increased the activities of several antioxidation enzymes in mice to different extents. CONCLUSION: Fraction F/H have the effect of antilipid peroxidation,may scavenge active oxygen free radical and increase the activity of SOD.