Use of membrane separation methods in the production of Clostridium perfringens toxoid

: The dynamics of ultrafiltration was found to depend on the composition of the nutrient medium in which C.perfringens toxin accumulated. A new composition of casein-pancreatic medium with the reduced content of high-molecular peptides ensuring a high effect of membrane separation was developed. The influence of the main factors (pressure, concentration, the number of diafiltration cycles) on the characteristics of the membrane separation of type A C.perfringens culture. The optimum conditions for the purification of C.perfringens toxin by ultrafiltration were established with due regard to the data obtained in this investigation. Toxin concentrates were found to contain a fraction inhibiting their sorption capacity, and the method of the elimination of the inhibiting component was developed. C.perfringens toxoids, considerably exceeding commercial preparations in specific activity and immunogenic potency, were obtained.