Purification,Identification of Human Telomerase and Its Protein Subunits

To purify human telomerase complex and analyse its protein subunit components,an affinity purification protocol using human telomere repeat sequence as affinity ligand was designed and carried out to purify HeLa cell telomerase.A DIG gel shift protocol was performed to identify the purified component.SDS PAGE was used to analyze protein subunits of purified component.The component with telomerase activity was obtained from elution fluid.A single band on native gel and a special DIG gel shif band on X ray film were observed.Four protein subunits were observed using SDS PAGE. Their relative molecule mass are 220 ku,212 ku,116 ku and 43 ku,respectively.Human telomerase complex and its 4 protein subuints were obtained.