A New type of Protein Profiling System using CPDIB (Crude Protein Direct Blotting) assay

Proteome research in the medical field is expected to accelerate the understanding of disease mechanism, and to create new therapeutic and diagnostic concept. For protein profiling, this paper proposes a new methodology named CPDIB (Crude Protein Direct Blotting). In CPDIB assay, crude protein sample is directly immobilized on a hydrophobic membrane, and then the expression of molecules in the sample are analyzed quantitatively by using a special device, where the membrane is used as a field of immune reaction. The over-all structure of the special device is based on the conventional slot-blot device. Mechanical improvement in the air-tightness of the case holding the membrane realizes the direct blotting and results in the high performance of the stability in the immune reaction. This paper shows the mechanical structure of the membrane assembly called ImmobiChip and the evaluation of the protein profiling and its reproducibility.