Differential expression of the cytokine receptors for human interleukin (IL)-12 and IL-18 on lymphocytes of both CD45RA+ and CD45RO+ phenotype from tonsils, cord and adult peripheral blood
2004
The objective of this study was to demonstrate the variable expression of cytokinc receptors on naive versus memory human CD4 T cell subpopulations in tonsillar tissue, cord blood and adult blood. We prove that the receptors for both interleukin (IL)-12 and IL-18 are expressed exclusively on memory T cells. This observation was seen not only on the CD45RO + memory T cells but also on a significant percentage of the CD45RA + , CD62L, CD27 and CCR7 populations. Furthermore, CD45RA + CD62L + , CD27 + or CCR7 + CD4 + T cells that expressed IL-12Rβ1and IL-18Rα did not express CD31, a marker for recent thymic emigrants. We reveal that cord blood lymphocytes do not express IL-12Rβ1 whereas IL-18Rα expression was detected at low levels. Importantly, the IL-12Rβ2 signalling chain, which is absent in all resting T cells, was up-regulated in both CD45RA + and CD45RO + T cells as a result of stimulation with anti-CD3 and anti-CD28 in vitro. This observed up-regulation was, however, restricted to 80% of the total CD4' population. Finally, a very small proportion of the CD4 CD45RO + tonsillar T cells expressed the IL-12 and IL-18 receptors, thereby establishing the differential expression of these receptors between peripheral and tonsillar memory T cell subpopulations.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
18
References
17
Citations
NaN
KQI