Characterization of Chicken Portomicron Remnant and Very Low Density Lipoprotein Remnant

Portomicron remnant and very low density lipoprotein (VLDL) remnant in chickens were prepared by the method using ultracentrifugation (d<1.019) following an in vitro incubation of portomicron or VLDL prepared from chicken plasma with chicken lipoprotein lipase (LPL) for 2h at 37°C. The portomicron remnants and VLDL remnants yielded the almost single peak in the elution pattern on Bio-Gel A50m column (1.6×50cm) and the particle sizes were smaller than portomicron and VLDL since the elution of remnants by Bio-Gel A50m was similar to LDL rather than portomicron and VLDL. Chicken remnants had low triacylglycerol contents with high cholesterol and lower apolipoprotein C contents compared to portomicron or VLDL. These findings present the possible procedure to prepare in vitro chicken LPL-generated portomicron- and VLDL-remnants and suggested that the chicken remnants are characterized by a peculiar apolipoprotein composition while resemble to mammalian remnants in the lipid composition and particle size.