Rapid detection of persistent foot-and-mouth disease virus infection in buffaloes by rt-lamp

Foot-and-mouth disease virus (FMDV) was detected from the oropharyngeal fluid (OPF) by reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay, SYBR Green dye based quantitative reverse transcription polymerase chain reaction (qRT-PCR) and conventional virus isolation for identification of carriers in infected bovines in Haryana. Out of the 11 OPF samples collected at 45 days post infection, RT-LAMP assay detected five as compared to three by qRT-PCR and two by virus isolation. Compared with qRT-PCR and virus isolation, RT-LAMP was consistently fast and amplification products were detected by visual inspection and agarose gel electrophoresis. The results indicated the potential usefulness of the RT-LAMP technique as a simple and rapid procedure that can be employed for the detection of FMDV carriers in OPF samples.