Metabolomics analysis of the effects of temperature on the growth and development of juvenile European seabass (Dicentrarchus labrax).

Abstract Temperature variations have significant impacts on the growth and development of fish. In this study, the effects of temperature on the growth and development of European seabass (Dicentrarchus labrax) were investigated using ultra-performance liquid chromatography–tandem mass spectrometry-based metabolomics. Three groups of fish were exposed to various temperatures for 60 days: T1-E (10°C), T2-E (15°C), and T3-E (20°C). Afterward, the temperature of all groups was increased to 20°C and maintained for 62 days (T1-S, T2-S, T3-S). The livers were extracted for subsequent analysis. In the first stage of the experiment, the growth rate was highest in the T3-E group, followed by the T1-E and T2-E groups. The following metabolites identified by comparative analysis were found to be elevated: L-thyroxine, cysteamine, uridine diphosphate (UDP)-glucose, α-ketoglutaric acid, carbamoyl phosphate, and guanidine acetic acid of the T1-E group. Pathway analysis of the altered metabolites suggested changes in glucose metabolism, arginine and proline metabolism, the tricarboxylic acid cycle, the ornithine cycle, histidine metabolism, and taurine metabolism, which were involved with growth and development. Meanwhile, partial compensatory growth was observed in fish in the T1-S and T2-S groups. Metabolites identified as potential markers of growth included L-cysteine, taurocholic acid, UDP-glucose, and L-thyroxine. The significantly changed metabolic pathways were cysteine and methionine metabolism, bile secretion, tyrosine metabolism, and hypotaurine metabolism. We screened out the marker metabolites and metabolic pathway could provide important insights into the potential mechanisms of temperature affects the growth and development of European seabass. All in all, our research can provide theoretical basis and technical guidance for efficiently culturing European seabass.