N-Terminal Amino Acid of Eel Hemoglobin

The 4 Hb components of eel were isolated and analyzed for N-terminal structure. Only 2 N-terminal valyl residues per molecule were detected by the DNP method for each component. On the other hand, the presence of 2 acetyl groups per mole-cule was demonstrated in all the components by paper and gas chromatography and by the colorimetry of ferric-hydroxamic acid complex derived. Based on those results, it may be concluded that each of the 4 Hb's has 2 valyl and 2 N-acetylated amino acids as N-termini.