Optogenetic Repressors of Gene Expression in Yeasts Using Light-Controlled Nuclear Localization
2019
Introduction
Controlling gene expression is a fundamental goal of basic and synthetic biology because it allows insight into cellular function and control of cellular activity. We explored the possibility of generating an optogenetic repressor of gene expression in the model organism Saccharomyces cerevisiae by using light to control the nuclear localization of nuclease-dead Cas9, dCas9.
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