Optimization of cultural parameters for lipase production by Bacillus subtilis Y-IVI

A B S T R A C T A bacterial strain isolated from oil contaminated soil and identified as Bacillus subtilis Y-IVI based on 16S rDNA analysis was screened for lipase production on tributyrin agar. Maximum lipase production was observed at 48 h of growth. Starch was preferred over other carbon sources for optimum production of lipase at a concentration of 1.5%. Peptone was found to be an ideal nitrogen source for production at a concentration of 1.5%. Among the natural oils, olive oil was able to induce more lipase production. Basal medium supplemented with tween 80 enhanced lipase production to a significant level.