Abortos por Neospora caninum em bovinos do sul de

2013 
Orlando D.R., Costa R.C., Soares B.A., Oliveira N.S.C., Nascimento L.C., Peconick A.P., Raymundo D.L. & Varaschin M.S. 2013. (Neospora caninum abortions in cattle in southern Minas Gerais, Brazil.) Abortos por Neospora caninum em bovinos do sul de Mi- nas Gerais. Pesquisa Veterinaria Brasileira 33(11):1332-1338. Setor de Patologia Veterina- ria, Universidade Federal de Lavras, Cx. Postal 3037, Lavras, MG 37200-000, Brazil. E-mail: msvaraschin@dmv.ufla.br The present study evaluates the participation of Neospora caninum in bovine abortions from farms in the southern region of Minas Gerais state, Brazil, through histopathologi- cal, immunohistochemical (IHQ) analysis and DNA detection by polymerase chain reaction (PCR). The material used in this study was obtained through retrospective study of bovine abortion cases received by the Setor de Patologia Veterinaria da Universidade Federal de Lavras and by necropsies performed in fetuses from 2011 to 2013. From 60 fetuses stu- died, 30 (50%) had microscopic lesions; 19 (63%) of them had histologic lesions compa- tible with N. caninum abortion, characterized by multifocal non-suppurative encephalitis, necrosis and gliosis in the central nervous system and multifocal nonsuppurative myocar- ditis and myositis. From the 19 fetuses with protozoa lesions in 14 the final diagnosis was performed; five were confirmated only by IHQ staining and five were positive only by PCR. Four fetuses were positive in IHC and in PCR. In five fetuses from the retrospective study lesions compatible with N. caninum were seen, but the presence of the protozoan was not confirmed by IHQ labeling. The findings demonstrate that N. caninum is an important agent associated with abortion in cattle from Minas Gerais. Therefore, beyond the microscopic lesions association between IHC and PCR was essential to confirm the diagnosis.
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