Study on the Separation,Purification and ImmunologicalActivity of the Polysaccharide from Leaves of Acanthopanacis Senticosi

Structural information and immunological activity water-soluble polysaccharide from leaves of Acanthopanacis Senticosi were investigated.Crude polysaccharide was obtained from leaves of A.Senticosi after removing protein by hot water extraction,ethanol precipitation and Sevag.A homogeneous polysaccharide,named ASP-2-1,was separated and purified from the crude polysaccharide through EDAE-Sepharose Fast Flow Ion-exchange chromatograph and Sephadex G-75 gel filtration chromatography.The monosaccharide components of ASP-2-1 were determined by capillary zone electrophoresis(CZE) after trifluoroacetolysis and l-phenyl-3-methyl-5-pyrazolone(PMP) derivatization in tendorn order. The purity and molecular weight of ASP-2-1 were determined by HPGPC.The immunological activity of ASP-2-1 was menstruated with spleen lymphocytes of mouse in vitro experiment.The ASP-2-1 whose molecular weight was 13.6 BCD was mainly composed of glucose,arabinose,galactose,fructose,rhamnose and xylose with the mole ratio of 27.33:8.92: 3.03:1:7.59:20.51.ASP-2-1 showed proliferating effect on splenic lymphocyte markedly.The ASP-2-1 from leaves of A. Senticosi had strong immunological activity in vitro.