Dynamics of Zebrafish Heart Regeneration Using an HPLC–ESI–MS/MS Approach

Failure to properly repair damaged due to myocardial infarction is a major cause of heart failure. In contrast to adult mammals, zebrafish hearts show remarkable regenerative capabilities after substantial damage. To characterize protein dynamics during heart regeneration, we employed an HPLC-ESI-MS/MS approach. Myocardium tissues were taken from sham operated fish and ventricle resected sample at three different time points (2 days, 7 days, and 14 days), dynamics of protein expression were analyzed by an ion current-based quantitative platform. More than two thousand protein groups were quantified in all 16 experiments. Two hundred and nine heart regeneration related protein groups were quantified and clustered into 6 time-course patterns. Functional analysis indicated that multiple molecular function and metabolic pathways were involved in heart regeneration. Interestingly, Ingenuity Pathway analysis revealed that P53 signaling was inhibited during the heart regeneration, which was further verified by Q...