Effect of Probiotics Supplementation in Laying Hens Diets on Performance and Nutrient Digestibility of hens at late Laying Stage

This experiment was conducted to evaluate the effect of Bacillus supplementation in laying hens diets on performance and nutrient apparent digestibility in the anaphase of laying production.A total of 864,50-week-old,healthy Hy-line brown laying hens were selected to carry out metabolize experiment using direct collection feces method.These layers were randomly divided into 4 groups with 8 repeats(3 layers in each repeat),included one control group fed a corn-soybean-cottonseed basal diet without any antibiotics and prebiotics and three experiment groups supplemented 0.01% Bacillus,0.02% Bacillus and 0.02% compound probiotics,respectively.Pre-feeding period lasted 4 days for achieving the optimum performance.The feces from birds of each repeat were collected continuously for 3 days when birds fully adapted to the diets and showed the best production performance.The utilization efficiency of apparent digestibility of energy(AME),crude protein(CP),dry matter(DM),Ca,P and amino acid in diets supplemented with different probiotics were determined.The results showed that 0.02% Bacillus and 0.02% compound probiotics supplementation could significantly improve laying rate,egg production and feed intake(P0.01).AME,DM and Ser,Glu,Thr,Gly,Arg,Ala,Pro,Val,Phe,Ile,Leu,His,Lys,Met,Cys in hens during the late laying period have been improved by 0.02% Bacillus and 0.02% compound probiotics supplementation(P0.05),significantly.0.02% compound probiotics supplementation could also significantly improve average egg weight(P0.05),lower feed conversion efficiency and increase of CP,Ca,P(P0.05).In conclusion,the both probiotics can significantly improve the performance and nutrient apparent digestibility of hens during the late laying period.