The expression of Drosha, DGCR8, Dicer and Ago-2 genes are upregulated in human umbilical vein endothelial cells under hyperglycemic condition
2018
Objectives. It has been shown that dysregulation of miRNAs expression contributes to the
pathogenesis and progression of the diabetes and diabetes-related complications. Drosha, DGCR8,
Dicer, and Ago-2 are involved in the miRNA maturation. The aim of the present study was to investigate
the mRNA expression levels of these genes in the human umbilical vein endothelial cells
(HUVECs) under hyperglycemic condition.
Methods. HUVECs were cultured in normo- (5 mM) and hyperglycemic (25 mM) conditions for
24 h. As osmotic control, cells were treated with D-mannitol (25 mM, for 24 h). The mRNA expression
levels of Drosha, DGCR8, Dicer and Ago-2 were evaluated using quantitative real-time PCR.
Results. The expression level of Drosha, DGCR8, Dicer, and Ago-2 were increased in hyperglycemic
HUVECs compared to the control group.
Conclusion. Our results show that under hyperglycemic condition, expression of genes involved
in the miRNA maturation was significantly increased in HUVECs. Upregulation of these
genes may have role in diabetic complications through the dysregulation of the miRNA expression.
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