Acetate inhibition on growth of recombinant E. coli and expression of fusion protein TGFaα-PE40

Acetate was inhibitory to the growth of early induced E. coli cells and their expression of fusion protein, transforming growth factor-α-Pseudomonas exotoxin 40 (TGFα-PE40), but the inhibitory level was strain dependent For E. coli JM109 (pTAC-TGF57-PE40), 2 g/L of added acetate (3 g/L of total acetate in the medium) decreased TGFa-PE40 production by 38.0%. Acetate was less inhibitory to E. coli RR1, and RR1 was not affected by adding 2 g/L of acetate. However, 5 g/L of added acetate (6.7 g/L of total acetate in the medium) decreased TGFα-PE40 production by 21.2%. These results indicate that higher acetate concentration was associated with inhibition of TGFα-PE40 expression of E. coli JM109 during late induction.