The Effects of Glutamine on Sucrase, Maltase, Lactase Activity and Spectrin, Clathrin Expression in Repairing Microvilli Ileum in Rats Malnutrition

Glutamine is the most abundant amino acid in the blood and plays a key role of intestinal response to local and systemic injuries suchas diarrhea and malnutrition. The purpose of this study was to explain the effect of oral glutamine on sucrase, maltase, lactase enzymeactivity and spectrin, clathrin expression also the correlation with ERK-1 and STAT-3 signaling pathways in repairing microvilliileum intestine in rat malnutrition. The method employed in this study was experimental research, with control samples, measurableand reliable, with the design of the study was post test only group design. showed that twenty growing male rats (aged 12 week)were divided into 4 groups control, malnutrition, control+glutamine and malnutrition+glutamine. For the fi rst 15 days experiment,animals in the test groups received a karak diet (low calorie diet of dried rice) for the next 15 days. Control and malnutrition groupsreceived either glutamine supplement with the doses 500 mg/kg body weight/day. On the 30th days, the animals were weighed andsacrifi ced of the intestine was taken and prepared for enzymatic and expression examination. Sucrase activity between malnutritionwith malnutrition+glutamine groups there indicated signifi cance ileum (p = 0.04). Maltase activity when compared malnutrition withmalnutrition+glutamine groups there were signifi cant differences in the ileum (p = 0.00). Lactase activity when compared betweenmalnutrition with malnutrition + glutamine groups there were signifi cant differences in ileum (p = 0.04). The expression of spectrinand clathrin were evidence enlarge of enterocyte in the normal group after being given glutamine compared to malnutrition groupafter being fed glutamine, spectrin and clathrin expression appearance on ileum. Spectrin and clathrin molecular weight protein in theileum with a molecular mass of 130-250 KDa. Relationship between glutamine and meaningful pathway signaling proteins ERK-1 inthe ileum (p = 0.03) and also signifi cant association between glutamine and STAT-3 in ileum (p = 0.00). The research proved that therewas an increase activity of sucrase, maltase, lactase, also spectrin and clathrin which have expression differences of each group. Thereare links between ERK-1 and STAT-3 signaling pathway in the repairing microvilli of rat ileum intestinal damaged by malnutrition andimproved after administration of oral glutamine.