2.組織細胞化学シリーズ(若手研究者へのヒント) 共焦点レーザー顕微鏡による形態,機能解析-マルチモード顕微鏡による癌細胞の形態,機能解析の実際(2)
2009
The confocal laser scanning microscope (CLSM) is a device for obtaining high-resolution optical images of immunofluorescent staining. The CLSM can produce in-focus images of thick specimens, a process known as optical sectioning. The images are reconstructed with a computer, using 3-dimensional image software, allowing 3-dimensional reconstructions of topologically complex objects. On the same tissue sections, the CLSM can obtain the images of differential interference contrast. Recently, a special inverted CLSM-the multimode microscopy system-has been used to examine the morphology and functions of cells. A multimode microscopy system can be used to obtain images of CLSM, total internal reflection fluorescence, time-lapse, and micromanipulation. In the present study, we show images of pancreatic cancer cells as an example.
Keywords:
- General surgery
- Microscope
- Analytical chemistry
- Microscopy
- Differential interference contrast microscopy
- Confocal
- Total internal reflection fluorescence microscope
- Optical sectioning
- Immunofluorescence
- Laser scanning
- Biology
- tissue sections
- Multi-mode optical fiber
- Confocal laser scanning microscopy
- Optics
- Surgery
- confocal laser scanning microscope
- Medicine
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
0
References
0
Citations
NaN
KQI