J006 Beta-adrenergic stimulation activates protein kinase C-epsilon through Epac in cardiomyocytes

Protein kinase C (PKC) activation is classically considered as independent of the β-adrenergic pathway. However, the cAMP-activated exchange factor Epac was recently shown to activate phospholipase C. β-A stimulation is thus likely to stimulate PKC. We evaluated in cardiomyocytes whether βA stimulation could activate PKCɛ. Rat neonatal cardiomyocytes were subjected to isoproterenol stimulation (ISO). Inositol trisphosphate production was increased by 50 % by 1 μm ISO (p