Comparative proteome analysis of the hippocampus injuried by electromagnetic radiation

Aim To investigate the difference of hippocampus proteome maps after radiation by three electromagnetic waves of different frequency.Methods Comparative proteomic approach based on two dimensional gel electrophoresis(2-DE),mass-spectrum identification and bioinformatics analysis was used to study the difference of hippocampus total protein at 6 h after electromagnetic radiation exposure.Western blot and RT-PCR technique was applied to detect the differential protein for further verification on the level of protein and gene respectively.Results The three kind of electromagnetic radiation changed the hippocampus proteome map similarly.45 differential protein spots were found by image analysis,among of which 26 spots were identified initially.For raf kinase inhibitor protein(RKIP),which was one of the differential proteins and correlated with signal transduction, Western blot and RT-PCR results further verificated 2-DE results.The expression of RKIP protein and mRNA was down regulated significantly at 6h after radiation,and no significant difference was observed between any radiation groups.Conclusion The injury mechanisms of hippocampus by the three kind of electromagnetic radiation possessed similarity,and the degree of injury showed frequency dependence.RKIP may play an important role in the hippocampus injury by radiation.