Biological Features of Aeromonas Hydrophila

T3 Aeromonas hydrophila was used as model strain to study the biological features of Aeromonas hydrophila.The growth of the strain on the BA and TSA solid medium is better than other solid medium,and the growths in TSB and BHI broth are better than other fluid medium.The growth curve indicates that the number of viable T3 Aeromonas hydrophila growing in the TSB broth reaches the peak after cultured 16~20 hours and the viable strain decreases rapidly after cultured 24 hours.The discovery of Aeromonas hydrophila growth character establishes the foundation of large-scale culture of the bacteria.Survival curve indicates that the strain preserved in physiologic saline,PBS and TSB broth at 4℃ can survive well at protophase but difficult survive at anaphase.Moreover spectro photometer assay of the strain solves the problem of counting of the viable strains which settles the foundation of animal experiment of Aeromonas hydrophila.The pathogenicity to china immature soft-shell turtle and johnny carp of the T3 Aeromonas hydrophila is researched and LD50 of T3 Aeromonas hydrophila to china immature soft-shell turtle and johnny carp are 8.40×108 CFU and 8.63×108 CFU respectively.