Genetic Diversity of Yellow-seeded Parental Lines of Brassica napus Using SSR Markers

Selection of suitable parents is one of the key works in hybridization of Brassica napus L.The genetic diversity of 180 parental germplasm lines of yellow-seeded B.napus provided by the Chongqing Rapeseed Engineering Research Center of Southwest University was analyzed.Three hundred and eight alleles were detected via 60 SSR primer pairs,which were selected from different linked groups in B.napus.Among them,207 polymorphic bands were obtained,accounting for 67.2% of the total bands observed.The UPGMA cluster analysis based on SSR data showed that these parental lines could be classified into three groups at the threshold of 0.566,which agrees with the pedigree analysis.This could also provide the theoretical basis for the hybridization and breeding of yellow-seeded B.napus.