Studies on Peptides. CXXXVIII. : Conventional Solution Synthesis of Bovine Hypothalamic Growth Hormone Releasing Factor (bGRF)

A 44 residue peptide amide corresponding to the entire amino acid sequence of bovine hypothalamic growth hormone releasing factor (bGRF) was synthesized by assembling nine peptide fragments via the azide followed by deprotection with 1M trifluoromethanesulfonic acid-thioanisole in trifluoroacetic acid. Met(O) was reduced by dimethylselenide during the above acid treatment. The synthetic peptide was as active as synthetic human GRF-44-NH2 in vitro assay.