Influence of therapy of regulating qi and activating blood circulation on gene expression proflle in rats with precancerosis of atrophic gastritis

Objective To observe the influence of TCM therapy of regulating qi and activating blood circulation on the gene expression profile of gastric mucosa in rats with precancerosis of atrophic gastritis. Methods The rat model of precancerosis of atrophic gastritis was established by the synthetic method of metal spring insertion and feeding of NaCI solution and heat paste.The changes of differential gene expressions in the rats were detected by using gene chip technology after the intervention of the TCM therapy.The differential expression genes were screened and then identified by applying RT-PCR. Results There were totally 14 differential expression genes screened,and among them 8 were up-regulated genes and 6 were down-regulated genes.All of them consisted of the differential expression gene profile after the intervention of the TCM therapy in rat with precancerosis of atrophic gastritis,among which ptk2 was regulated significantly downward in the spontaneous recovery group and regulated significantly upward after the intervention of the TCM therapy.Conclusion The TCM therapy of regulating qi and activating blood circulation can regulate upward the gene expression of ptk2 in order to regulate the remodeling of actin cytoskeleton,and improve the repairing and healing of gastric mucosa.It is may be one of the mechanisms of the TCM therapy for treating precancerosis of atrophic gastritis.