Lethal anaphylactic shock model induced by human mixed serum in guinea pigs

Objective To establish an anaphylactic shock model induced by human mixed serum in guineapigs. Methods Eighteen guinea pigs were divided into two groups: sensitized and control , The sensitizedgroup were immunized intracutaneously with human mixed serum and then induced by endocardiac injec-tionafter 3 weeks. Results Symptoms of anaphylactic shock appeared in the sensitized group. The level ofserum IgE were increased in the sensitized group significantly . Conclusions An animal model of ana-phylactic shock wer established successfully. It provide a tool for both forensic study and anaphylacticshock therapy.