Neuroprotective Effect of Ultrasound Neuromodulation on Kainic Acid-Induced Epilepsy in mice.

Preliminary evidence suggest that low intensity pulsed ultrasound (LIPUS) has neuroprotective effects on ischemic stroke, d epression and other conditions leading to neuronal cell death (e.g., Pa rkinson's disease). The purpose of this study was to investigate the n europrotective effects of LIPUS in epileptic mice. Methods Mice we re made epileptic through Kainic Acid (KA) administration and then stimulated with LIPUS. The neuroprotective effect of ultrasound was evaluated by observing the latency, anxiety-like behavior, and levels of proteins related to inflammation, apoptosis, or signaling pathways. The safety of LIPUS was assessed by hematoxylin and eosin (HE 1 kHz PRF = 7.00 ± 0.31 days), improved the anxiety -like behavior, and inhibited the expression of inflammatory factors and apoptosis-related proteins. Additionally, H&E and Nissl staining results confirmed that LIPUS did not damage the brain. Conclusion These findings suggest that LIPUS has neuroprotective effects in mic e with KA-induced epilepsy. Significance LIPUS may offer a new th erapeutic approach to epilepsy.