Analysis of Genetic Diversity Among Natural Hybrid Progenies of Camellia sinensis‘Huangshanzhong’

Genetic diversity among natural hybrid progenies of Camellia sinensis ‘Huangshanzhong’ was studied,using some morphological traits and ISSR markers.The average coefficient of variation and Shannon-weave index were 20.5%(ranged between 12.5% and 33.2%)and 1.89(ranged between 1.60 and 2.07)respectively,based on investigation of morphological traits.Using 22 ISSR primers,402 discernible DNA fragments were generated,among which 396(98.5%)were polymorphic.The mean of poly-morphism information content(PIC)for the ISSR primers was 0.90.The mean of Nei's genetic diversity(H) and Shannon's information index(I) were 0.32 and 0.49 respectively,among all tested individuals.The results showed that genetic variation was high among those progenies of C.sinensis‘Huangshan-zhong’.The relationship between morphological and ISSR-based distance was significant positively correlated(r = 0.61,P = 0.01)by Mantel testing.It indicate that ISSR is a useful tool for relationship analysis of tea germplasm resources.Based on morphological and ISSR data,several plants were clusertedinto a major group,and only few individual plants were divided into single group.These special plants would be of great help to the tea breeding in future.