Genomic characterization of non-small cell lung cancer by targeted massively parallel sequencing in African Americans.

8086 Background: Technological advances have enabled the comprehensive analysis of genetic perturbations in non-small cell lung cancer (NSCLC), but African-Americans (AA) have often been underrepresented. This ethnic group presents higher lung cancer incidence and mortality, and some studies have suggested a lower incidence of EGFR mutations. Herein we used a targeted massively parallel sequencing approach to assess NSCLC samples resected from AAs. Methods: We designed and validated a NSCLC-specific panel comprising 80 genes of potential interest for therapy. Libraries were constructed using a custom Agilent Haloplex kit, and sequenced on an Illumina HiSeq 2500. Cell lines and clinical formalin-fixed paraffin-embedded samples with known mutation status were used for validation. NSCLC archival tissues derived from AAs were retrospective collected, and medical charts were reviewed to assess clinical data. ALK translocations were evaluated by fluorescence in situ hybridization (FISH). Results: Targeted captu...