Validation Of M.I.C.Evaluator Strips For Testing Antimicrobial Susceptibilities Of Fastidious Organisms According To EUCAST Guidelines

Microtitre plates containing cation adjusted Thermo ScientificTM Mueller-Hinton Broth (CAMHB) supplemented with 5% Lysed Horse Blood, 20mg/L β-NAD and appropriate concentrations of each antimicrobial agent (ceftriaxone, clindamycin, ciprofloxacin, erythromycin, imipenem, linezolid, levofloxacin, meropenem, teicoplanin, tetracycline and vancomycin), were inoculated with a 0.5 McFarland inoculum suspension of each Streptococci and Haemophilus isolate (to achieve an inoculum level of 7.5x105CFU/ml) using a multi-channel pipette, as shown in Figure 1. All microtitre plates were incubated in aerobic conditions at 35±1 C for 16-20 hrs according to EUCAST guidelines.