Investigating the Antimicrobial Activities of the Biosynthesized Selenium Nanoparticles and Its Statistical Analysis

The present investigation concentrates on the green combination of biosynthesized selenium nanoparticles utilizing aqueous extract of cow urine as a green technique with no external chemicals, reagents, or surfactants. The combination of parameters, for example, the sodium selenite precursor concentration, or the concentration of cow urine extract, time of synthesis, and pH on the particle size of combined selenium particles, was explored and enhanced. The participation of the factors in controlling the particle size of reduced SeNPs was quantitatively evaluated by means of analysis of variance (ANOVA). The UV-Vis spectroscopy was utilized to screen the selenium nanoparticle development followed by electron microscopy (SEM and TEM), DLS, AFM, and EDAX. The optimized nanoparticles were subjected to antibacterial activity against Gram-positive and Gram-negative cultures, out of which the highest activity was observed for Klebsiella sp. The biosynthesized and optimized selenium nanoparticles can be further used for various pharmaceutical or industrial applications.