Síntesis Enzimática de Alcamidas en Sistemas No Acuosos y Ensayo de su Bioactividad In Vitro

RESUMEN. Se sintetizaron diez moleculas de alcamidas usando la enzima Novozym lipasa B de Candida antartica en tolueno anhidro. Como sustrato de la reaccion se utilizaron acidos grasos y esteres de acidos grasos en presencia de isobutilamina o feniletilamina. El equilibrio de las reacciones se alcanzo entre 24 y 48 h, con niveles de conversion de 35,9 a 99,2%, obteniendose mayor efectividad de reaccion utilizando feniletilamina. Los ensayos de bioactividad de ocho de las alcamidas obtenidas, sobre el crecimiento de bacterias Gram(+) reflejaron que las alcamidas con el sustituyente feniletil, son mas activas que con el isobutilo. Los ensayos frente a bacterias Gram(-), no manifestaron inhibicion. SUMMARY. “Enzymatic Synthesis of Alkamides in Non-aqueous Systems and In Vitro Assay of Bioactivity” . Ten molecules of alkamides were synthesized using the Novozym lipase B from Candida antarctica in anhydrous toluene. Fatty acids and fatty acid esters and isobutylamine or phenylethylamine to compare their efficiency as nucloephiles. The reaction equilibrium of was achieved between 24 and 48 h, with conversion levels between 35.9 and 99.2%. Higher effectiveness was observed using phenylethylamine as nucleophyle. Bioactivity assays of eight obtained alkamides on development of Gram(+) bacteria displayed better performance when the amide group was substituted with phenylethylamine than with isobutylamine. Assays with Gram(-) bacteria did not show an inhibitor effect.