IN VITRO 3D СИСТЕМЫ КАК АЛЬТЕРНАТИВА ОПУХОЛЕВЫМ МОДЕЛЯМ IN VIVO

New models of tumor growth, as an alternative to animal xenografts, play an important role in pre-clinical studies. Substrate-independent 3D multicellular tumor structures are the adequate models of malignant node formation at the avascular growth phase. We performed a comparative analysis of proliferative parameters for breast cancer cells MCF-7 in 2D and 3D culture during prolonged incubation under the condition of starvation. We discovered that survival and resistance to the adverse factors in 3D culture were higher than in 2D culture under the condition of substrates deficiency. Therefore, 3D culture is an adequate model to study cytotoxic and antiproliferative signals in evaluation of substances with antitumor properties. References 1.   Mueller-Klieser W. / Crit. Rev. Oncol./Hematol. 2000.V. 36. P.123–139. 2.    Mazzoleni G., Di Lorenzo D., Steimberg N. / Genes Nutr. 2009. V. 4. P.13-22. 3.    Kunz-Schughart L.A., Freyer J.P., Hofstaedter F., Ebner R.   / J. Biomol. Screen. 2004.  V.9. P.273–285 4.    Fracasso G., Colombatti M. / Crit. Rev. Oncol./Hematol. 2000. V.36. P.159–178. 5.    Madsen S.J, Sun C.H., Tromberg B.J., Cristini V., De Magalhaes N., Hirschberg H. / Lasers Surg. Med. 2006. V.38. P.555–564. 6.    Ostrovska L., Garmanchuk L. / Bull. Kiev Nat. Univ. Taras Shevchenko . Ser. Biol. 2013. V.2.No. 64. P.53-57. 7.    Breast cancer: prevention and control /World Health Organization. 8.    Ivascu A., Kubbies M. / Int. J. Oncol. 2007. V.31. P.1403-1413 9.    Kim J., Stein R., O'Hare M.  / Breast Canc. Res. Treat. 2004. V.85. P.281–291. 10.    Kelm J.M., Timmins N.E., Brown C.J., Fussenegger M., Nielsen L.K. / Biotechnol. Bioeng. 2003 V.83. No. 2. P.173-80. 11.    Garmanchuk L.V., Perepelytsina O.M., Sidorenko M.V.,  Ostapchenko L.I. / Cytol. Gen. 2009. V.43. No.5. P.305-309 12.    Arundel C.M., Leith J.T. / Int. J. Radiat. Oncol. Biol. Phys. 1986.  V.12. No.1. P.59-67. 13.    Mosmann T. / J. Immunol. Methods. 1983. V.65. No.1-2. P. 55-63. 14.    Nicoletti I. Migliorati G., Pagliacci M.C. / J. Immunol. Methods. 1991. V.139. No. 2. P.271-80.