An in planta technique for cis-/trans-stereochemical analysis of jasmonoyl isoleucine.

Abstract A novel technique for determining the cis -/ trans -stereochemistry of jasmonoyl-isoleucine by coupling its alcoholic derivatives by sodium borohydride with high performance liquid chromatography–tandem mass spectrometry is described. Resolving cis - and trans -stereochemistry of the jasmonates in Achyranthes plants exposed to airborne (exogenous) trans- d 2 MeJA was demonstrated as an example. This novel application firmly establishes for the first time that trans- d 2 MeJA is converted exclusively into trans -JA-Ile in Achyranthes leaves, whereas the subsequent de novo biosynthesized JA-Ile possesses cis -stereochemistry. The method is simple, reproducible and could be employed for in vivo cis -/ trans -stereochemistry analysis of jasmonates in plants.