Biotransformation of 4-methyl-7-allyloxy Coumarin by Transgenic Hairy Roots of Polygonum multiflorum

Objective:To synthesis 4-methyl-7-allyloxy coumarin by Williamson etherification from 4-methyl-7-hydroxy coumarin and apply as a substrate in hairy roots of Polygonum multiflorum.Methods:The synthesis reaction of 4-methyl-7-allyloxy coumarin used the allyl bromide and potassium carbonate as catalysts,and acetone as solvent reacted for 17 hours,then the product was isolated.4-methyl-7-allyloxy coumarin was added into the media of supension transgenic hairy roots of Polygonum multiflorum which had been pre-cultured for 8 d,and then co-cultured for another 7 d.The biotransformation products were detected by TLC and HPLC and isolated by various chromatographic methods.Results:Two biotransformation products,4-methyl-7-hydroxy coumarin and 4-methyl-coumarin-7-O-β-D-glucoside were isolated and identified.Conclusion:Hairy roots of Polygonum multiflorum contains not only glycosyltransferase but also hydrolysis enzymes.