Various aspects of regulation of the biosynthesis of novobiocin and exoproteases in cultures of Streptomyces spheroides 35

Streptomyces spheroides 35, a novobiocin-producing organism, was used in the study. In addition to the antibiotic the culture produces proteases capable of splitting fibrin. Depending on the medium composition and component ratio different amounts of novobiocin and proteases were formed. To show various aspects of the biological interaction of the synthesis of proteolytic enzymes and novobiocin, the studies were performed with chloramphenicol. It was found that the main quantity of free amino acids accumulated in the culture liquid phase, while in the mycelial phase only traces of the amino acids were detected. The regularities of the free amino acid accumulation in the media with novobiocin and chloramphenicol were identical as compared to the control. The excess of novobiocin during the second phase of the culture development was shown to influence the synthesis of nucleic acids, whereas inhibition of protein synthesis was secondary.