Effect of titanium dioxide nanoparticles on hemogram in rats with gastric ulcer

Objective To explore the effect of titanium dioxide ( TiO2 ) nanoparticles on hemogram in rats with gastric ulcer.Methods Physicochemical properties of TiO2 nanoparticles were characterized.Twenty-four clear class SD male rats,aging 8 week-old,were randomly divided into 4 groups,6 rats for each group.20％ acetic acid were injected into the rats' stomach on the border of gastric body and pyloric antrum,and herebv established the gastric ulcer model. The rats in 4 groups were exposed to TiO2 nanoparticles through intragastric administration at 0,10,50 and 200 mg/kg body weight respectively for 30 days. Afterwards, the rats were conducted blood routine test and blood coagulation test for analysis.Results TiO2 nanoparticles were anatase crystals,closely spherical shape,whose average grain diameter was (75 ± 15) nm. The levels of white blood cell (WBC) count ( (8.48 ±3.28) × 109/L),lymphocyte ( LYM ) ( (6.85 ±2.53) × 109/L),moncyte (MOD) ( (0.27 ±0.12) × 109/L),granulocyte (GRN) ((1.37 ±0.86) × 109/L),red blood cell (RBC) ((8.20 ±0.49) × 109/L) and hematocrit (HCT) ( (45.3 ± 1.4 )％ ) in the 200 mg/kg dose group were significantly higher than those in the control group ((2.63±0.34) × 109/L,(2.25 ±0.26) × 109/L,(0.05 ±0.06) × 109/L,(0.33 ±0.26) ×109/L,(4.87 ± 2.37) × 109/L and (27.2± 13.3)％,respectively;t values were - 3.449,- 3.825,-3.554,-3.097,-2.972 and -2.936 respectively,P values all ＜0.05).The levels of WBC( (6.88 ±3.06) ×109/L),MOD ((0.20±0.07) ×109/L),RBC ((7.79±0.48) × 109/L) and HCT ((42.7±2.8 )％ ) in 50 mg/kg dose group were also statistically higher than those in the control group (t values were - 2.507,- 2.367,- 2.605 and - 2.511 respectively,all P values ＜ 0.05 ).There was no statistically difference found in other blood routine index and coagulation index between the three experimental groups and control group.Conclusion The long term intake of TiO2 nanoparticles caused a statistically increase in the amount of WBC and RBC in rats with gastric ulcer; however,there was no obvious changes found in blood platelet and coagulation index. Key words: Nanostructures; Rats, Sprague-Dawley; Stomach ulcer; Hematologic tests; TiO2 nanoparticles