ВОССТАНОВЛЕНИЕ СНА ПОСЛЕ ЕГО ТОТАЛЬНОЙ ДЕПРИВАЦИИ В УСЛОВИЯХ ДЛИТЕЛЬНОГО ДЕФИЦИТА СТРЕСС-ИНДУЦИБЕЛЬНОГО ШАПЕРОНА Hsp70, "Российский физиологический журнал им. И.М. Сеченова"

According to the modern hypotheses, 70 kDа Heat Shock Proteins (HSP70) play а role in sleep recovery, and sleep itself promotes recovery of neurons in preparation to subsequent wakefulness. The study aims to determine whether the stress-inducible Hsp70 chaperone is involved in sleep recovery after total sleep deprivation. Here we applied RNA-interference in order to suppress the synthesis of Hsp70 directly in the ventrolateral preoptic area of the hypothalamus in rats. This approach allowed us to show for the first time that long-term decrease in Hsp70 level in the preoptic area leads to the lower rebound of slow-wave sleep after total sleep deprivation. Sleep rebound is essential for the restoration of neuronal structure and functioning after stress in sleep-deprived animals. These results imply the connection between Hsp70 in the «sleep regulation centre» in the preoptic area and molecular mechanisms of the sleep-wake switching.