The effect of vasoactive substances on synthesis and release of C-type natriuretic peptide in cultured human endothelial cells

AIM: To investigate the effect of endothelin (ET), angiotensin II (AngII) and homocysteine (Hcy) on C-type natriuretic peptide (CNP) synthesis and release. METHODS: Human endothelial cell was cultured; CNP was measured by radioimmunoassay method. RESULTS: ET and AngII could augment CNP synthesis in human endothelial cells. Compared with control group, 10 -9 ,10 -8 ,10 -7 mol/L ET and Ang II increased CNP content of endothelial cells by 1%( P 0.05), 49%( P 0.05),117%( P 0.01) and 137% ( P 0.01),165%( P 0.01),201%( P 0.01),respectively. A great dose of ET and Ang II also stimulated CNP release from cultured human endothelial cells. Hcy had no effect on CNP synthesis, but 10 -9 ,10 -8 ,10 -7 mol/L Hcy enhanced CNP release from cultured human endothelial cells by 17%( P 0.05),84%( P 0.01) and 555%( P 0.01), respectively. CONCLUSION: ET, AngII and Hcy might be involved in the synthesis and release of human endothelial cell CNP.